Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75578

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-75578
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU53-31

Concentration

1 mg/ml

View all Muscle Phosphofructokinase/PFKM Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Muscle Phosphofructokinase/PFKM/PFK-1 aa 107-156 / 780. (SwissProt: P08237 Human; SwissProt: P47857 Mouse; SwissProt: P47858 Rat)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31)

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Western blot analysis of Muscle Phosphofructokinase/PFKM/PFK-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: PC-3M cell lysate
Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578]

Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578]

Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578]

Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578]

Immunohistochemistry-Paraffin: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31) [NBP2-75578] - Analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
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Applications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Muscle Phosphofructokinase/PFKM

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (JU53-31)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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