Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Muscle Phosphofructokinase/PFKM Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 730 to 780 of human Phosphofructokinase, Muscle using the numbering given in entry NP_000280.1 (GeneID 5213).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for IP at 6 ug per reaction. For blotting immunoprecipitated PFK-1, NBP2-32170 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-32170] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PFK-1 antibody NBP2-32170 used for WB at 0.1ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Muscle Phosphofructokinase/PFKM

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

UniProt

NP_000280.1 (Human)

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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