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MX2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81018

Novus Biologicals, part of Bio-Techne
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NBP1-81018
NBP1-81018-25ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PYRRRSQFSSRKYLKKEMNSFQQQPPPFGTVPPQMMFPPNWQGAEKDAAFLAKDFNFLTLNNQPPPGNRSQPRAMG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MX2 Antibody

Western Blot: MX2 Antibody [NBP1-81018]

Western Blot: MX2 Antibody [NBP1-81018]

Western Blot: MX2 Antibody [NBP1-81018] - MX2 is localized to mitochondria in cultured cells. Western blotting of four distinct cell lines comparing endogenous MX2 expression under control conditions vs. treatment with 1,000 IU/ ml-1 of IFN-alpha-2alpha. HeLa, Hep3B and HepG2 cells induced MX2 expression by IFN treatment, but not Huh7 cells. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-020-14727-w), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: MX2 Antibody [NBP1-81018]

Immunohistochemistry-Paraffin: MX2 Antibody [NBP1-81018]

Immunohistochemistry-Paraffin: MX2 Antibody [NBP1-81018] - Staining of human spleen shows strong positivity in nuclear membrane in cells in red pulp.
Western Blot: MX2 Antibody [NBP1-81018]

Western Blot: MX2 Antibody [NBP1-81018]

Western Blot: MX2 Antibody [NBP1-81018] - Human glioma U87 cell lysate. WB image submitted by a verified customer review.

Applications for MX2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in literature (PMID: 300848227).

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

Reactivity reported in (PMID: 30333168), and from a verified customer review
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-81018 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MX2

MX2 is encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq]

Alternate Names

human interferon-regulated resistance GTP-binding protein MXB10Interferon-regulated resistance GTP-binding protein MxB, interferon-induced GTP-binding protein Mx2, MXB, myxovirus (influenza virus) resistance 2 (mouse), myxovirus (influenza) resistance 2, homolog of murine, Myxovirus resistance protein 2, p78-related protein, second interferon-induced protein p78

Entrez Gene IDs

4600 (Human)

Gene Symbol

MX2

UniProt

Additional MX2 Products

Product Documents for MX2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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