Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G12

Concentration

1 mg/ml

View all MxA/Mx1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MX1 (NP_002453) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse) (MX1), transcript variant 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MxA/Mx1 Antibody (OTI2G12)

Western Blot: MxA/Mx1 Antibody (OTI2G12) [NBP1-47859]

Western Blot: MxA/Mx1 Antibody (OTI2G12) [NBP1-47859]

Western Blot: MxA/Mx1 Antibody (2G12) [NBP1-47859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1.
Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (OTI2G12) [NBP1-47859]

Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (OTI2G12) [NBP1-47859]

Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody (2G12) [NBP1-47859] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MX1.
Western Blot: MxA/Mx1 Antibody (OTI2G12) [NBP1-47859]

Western Blot: MxA/Mx1 Antibody (OTI2G12) [NBP1-47859]

Western Blot: MxA/Mx1 Antibody (2G12) [NBP1-47859] - Analysis of extracts (10ug) from 1 cell lines by using anti- MX1 monoclonal antibody at 1:200 dilution
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Applications for MxA/Mx1 Antibody (OTI2G12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MxA/Mx1

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. Two transcript variants encoding the same protein have been found for this gene.

Long Name

Interferon-regulated Resistance GTP-binding protein MxA

Alternate Names

IFI-78K, IFI78, Mx1

Entrez Gene IDs

4599 (Human)

Gene Symbol

MX1

Additional MxA/Mx1 Products

Product Documents for MxA/Mx1 Antibody (OTI2G12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MxA/Mx1 Antibody (OTI2G12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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