Myc Epitope Tag Antibody (2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43627
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Myc Epitope Tag Antibody (2)
Immunogen
The immunogen used to generate this antibody corresponds to Myc Epitope Tag
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Myc Epitope Tag Antibody (2)
![Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]](https://resources.bio-techne.com/images/products/Myc-Epitope-Tag-Antibody-2-Western-Blot-NBP2-43627-img0004.jpg "Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]")
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Myc tag antibody [0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [0002] . (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected. EasyBlot anti-Mouse IgG Kit was used in Western blot.![Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]](https://resources.bio-techne.com/images/products/Myc-Epitope-Tag-Antibody-2-Immunocytochemistry-Immunofluorescence-NBP2-43627-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]")
Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]
Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Myc tag stained by Myc tag antibody [0002] (NBP2-43627) diluted at 1:2000.
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] -
Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc Epitope Tag antibody [GT0002] (NBP2-43627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Myc Epitope Tag Antibody (2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:2000
Immunoprecipitation
1:100-1:500
Western Blot
1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Myc Epitope Tag
Alternate Names
BHLHE39, Class E basic helix-loop-helix protein 39 (bHLHe39), Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64
Additional Myc Epitope Tag Products
Product Documents for Myc Epitope Tag Antibody (2)
Product Specific Notices for Myc Epitope Tag Antibody (2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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