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Myc Epitope Tag Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43691

Novus Biologicals, part of Bio-Techne
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NBP2-43691

Key Product Details

Species Reactivity

Epitope Tag

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide corresponding to a region within amino acids 410 and 419 of human c-Myc

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myc Epitope Tag Antibody

Western Blot: Myc Epitope Tag Antibody [NBP2-43691]

Western Blot: Myc Epitope Tag Antibody [NBP2-43691]

Western Blot: Myc Epitope Tag Antibody [NBP2-43691] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Myc tag antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody [NBP2-43691]

Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody [NBP2-43691]

Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody [NBP2-43691] - Analysis of paraformaldehyde-fixed Myc tag transfected HeLa, using Myc tag antibody (green) at 1:3000 dilution. Alpha-tubulin filaments were labeled (red) at 1:2500.
Western Blot: Myc Epitope Tag Antibody [NBP2-43691]

Western Blot: Myc Epitope Tag Antibody [NBP2-43691]

Western Blot: Myc Epitope Tag Antibody [NBP2-43691] - Analysis of extracts from untransfected control cells and transfected cells overexpressing Myc tagged protein, using Myc tag antibody. A: Non-transfected 293T lysate (10 ug) B: Transfected 293T lysate (10 ug) (~60 kDa) .

Applications for Myc Epitope Tag Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:10000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myc Epitope Tag

Alternate Names

BHLHE39, Class E basic helix-loop-helix protein 39 (bHLHe39), Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64

Additional Myc Epitope Tag Products

Product Documents for Myc Epitope Tag Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myc Epitope Tag Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.