Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08534
![Immunohistochemistry-Paraffin: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534]](https://resources.bio-techne.com/images/products/Myelin-PLP-Antibody-PLP1-4259-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08534-img0001.jpg "Immunohistochemistry-Paraffin: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Reptile
Applications
Flow Cytometry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # PLP1/4259
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free
Immunogen
Synthetic peptide GRGTKF that recognizes the C terminal region (aa272-277) of Myelin PLP protein. (Uniprot: P60201)
Reactivity Notes
Tenerife Lizard
Localization
Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07603).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free
Immunohistochemistry-Paraffin: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534]
Immunohistochemistry-Paraffin: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534] - Formalin-fixed, paraffin-embedded human brain stained with Myelin PLP Mouse Monoclonal Antibody (PLP1/4259).
Flow Cytometry: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534]
Flow Cytometry: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534] - Total extracellular vesicles with Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] (Catalog # NBP3-08534AF647) (left). Gate determined vesicles positive for PLP1 antibody ranging from 500-800nm size. Image on the right represents the same sample without antibody. 10ul of pre-isolated extracellular vesicles were incubated with 2ul of Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] , during 20 minutes at room temperature, and analyzed in nanoscale flow cytometer able to resolve particles scattering as little as 100um. Image from a verified customer review.Applications for Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Validated for Flow Cytometry from a verified customer review.
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Myelin PLP
Long Name
Proteolipid Protein
Alternate Names
DM-20, Lipophilin, MMPL, PLP1, SPG2
Gene Symbol
PLP1
Additional Myelin PLP Products
Product Documents for Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free
Product Specific Notices for Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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