Myelin PLP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1608
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the Myelin PLP gene product shared between the mouse (P60202) and human (NP_000524) protein sequences.
Marker
Myelin Marker
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Myelin PLP Antibody
![Immunocytochemistry/ Immunofluorescence: Myelin PLP Antibody [NB100-1608]](https://resources.bio-techne.com/images/products/Myelin-PLP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1608-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Myelin PLP Antibody [NB100-1608]")
Immunocytochemistry/ Immunofluorescence: Myelin PLP Antibody [NB100-1608]
Immunocytochemistry/Immunofluorescence: Myelin PLP Antibody [NB100-1608] - Immunohistochemistry photomicrograph of PLP immunoreactivity (Green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. blue is DAPI nuclear counterstain.Applications for Myelin PLP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000-1:5000
Immunohistochemistry
1:2000 - 1:5000
Western Blot
1:2000 - 1:5000
Application Notes
Western Blot reactivity reported in scientific literature (PMID: 24057669).
Reviewed Applications
Read 1 review rated 5 using NB100-1608 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
10mM PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Myelin PLP
Long Name
Proteolipid Protein
Alternate Names
DM-20, Lipophilin, MMPL, PLP1, SPG2
Gene Symbol
PLP1
UniProt
Additional Myelin PLP Products
Product Documents for Myelin PLP Antibody
Product Specific Notices for Myelin PLP Antibody
Chicken products cannot be exported to Canada.
Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 ug/ml, and the preparation was filter sterilized.
Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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