Myeloid Cell Marker Antibody (BM-1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32880
![Immunohistochemistry-Paraffin: Myeloid Cell Marker Antibody (BM-1) [NBP2-32880]](https://resources.bio-techne.com/images/products/Myeloid-Cell-Marker-Antibody-BM-1-Immunohistochemistry-Paraffin-NBP2-32880-img0001.jpg "Immunohistochemistry-Paraffin: Myeloid Cell Marker Antibody (BM-1) [NBP2-32880]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BM-1
Concentration
0.2 mg/ml
Product Summary for Myeloid Cell Marker Antibody (BM-1)
Immunogen
Human peripheral blood mononuclear cells
Localization
Cytoplasmic and nuclear
Specificity
Recognizes 183kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This monoclonal antibody reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.
Marker
Macrophage / Granulocyte Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
183 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34562)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Myeloid Cell Marker Antibody (BM-1)
Immunohistochemistry-Paraffin: Myeloid Cell Marker Antibody (BM-1) [NBP2-32880]
Immunohistochemistry-Paraffin: Myeloid Cell Marker Antibody (BM-1) [NBP2-32880] - Formalin-fixed, paraffin-embedded human Tonsil stained with Myeloid Specific Monoclonal Antibody (BM-1).Applications for Myeloid Cell Marker Antibody (BM-1)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Myeloid Cell Marker
Alternate Names
myeloid specific antigen, myeloid specific protein, p183 DNA-binding cytoplasmic ptotein
Additional Myeloid Cell Marker Products
Product Documents for Myeloid Cell Marker Antibody (BM-1)
Product Specific Notices for Myeloid Cell Marker Antibody (BM-1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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