Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26845

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14976

Format

BSA Free

Concentration

.6 mg/ml

View all Myeloperoxidase/MPO Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P05164, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

Myeloperoxidase/MPO Antibody (CL14976)

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (CL14976) [NBP3-26845]

Analysis in human bone marrow and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Myeloperoxidase/MPO Antibody (CL14976)

Western Blot: Myeloperoxidase/MPO Antibody (CL14976) [NBP3-26845]

Western blot analysis in human cell line NB4.
Myeloperoxidase/MPO Antibody (CL14976)

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (CL14976) [NBP3-26845]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
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Applications for Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myeloperoxidase/MPO

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Alternate Names

MPO

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myeloperoxidase/MPO Antibody (CL14976) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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