Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44266

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # CL14991

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Myeloperoxidase/MPO Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P05164, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free

Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody [NBP3-44266]

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody [NBP3-44266]

Staining of human bone shows strong cytoplasmic positivity in hematopoietic cells.
Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody [NBP3-44266]

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody [NBP3-44266]

Analysis in human bone marrow and skeletal muscle tissues using NBP3-44266 antibody. Corresponding Myeloperoxidase/MPO RNA-seq data are presented for the same tissues.
Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody [NBP3-44266]

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody [NBP3-44266]

Staining of human kidney shows no positivity in cells in tubules and glomerulus as expected.
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Applications for Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myeloperoxidase/MPO

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Alternate Names

MPO

Gene Symbol

MPO

Additional Myeloperoxidase/MPO Products

Product Documents for Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myeloperoxidase/MPO Antibody (CL14991) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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