MYH6 Antibody (CL2162)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36746

Novus Biologicals, part of Bio-Techne
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NBP2-36746
NBP2-36746-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2162

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MYH6 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:QVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEA

Epitope

RELEEISERLEEA

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MYH6 Antibody (CL2162)

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746] - Staining in human heart muscle and liver tissues. Corresponding MYH6 RNA-seq data are presented for the same tissues.
Western Blot: MYH6 Antibody (CL2162) [NBP2-36746]

Western Blot: MYH6 Antibody (CL2162) [NBP2-36746]

Western Blot: MYH6 Antibody (CL2162) [NBP2-36746] - Lane 1: Marker [kDa] Lane 2: Human skeletal muscle tissue lysate
Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2162) [NBP2-36746] - Staining of human heart muscle shows very strong cytoplasmic positivity in cardiomyocytes.
View 2 more images

Applications for MYH6 Antibody (CL2162)

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-36746 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYH6

Skeletal muscle Myosin or Myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Long Name

Myosin Heavy Chain 6, Cardiac Muscle, Alpha

Alternate Names

alpha-MHC, ASD3, CMD1EE, CMH14, MyHC-Alpha, MYHCA, Myosin-6, SSS3

Gene Symbol

MYH6

UniProt

P13533

Additional MYH6 Products

Product Documents for MYH6 Antibody (CL2162)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MYH6 Antibody (CL2162)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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