MYL9 Antibody (4C9E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16691

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16691-100ul
NBP3-16691-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4C9E8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MYL9 Antibodies »

Product Summary for MYL9 Antibody (4C9E8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MYL9 (P24844). MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYL9 Antibody (4C9E8)

Western Blot: MYL9 Antibody (4C9E8) [NBP3-16691]

Western Blot: MYL9 Antibody (4C9E8) [NBP3-16691]

Western Blot: MYL9 Antibody (4C9E8) [NBP3-16691] - Western blot analysis of extracts of various cell lines, using MYL9 Rabbit mAb (NBP3-16691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for MYL9 Antibody (4C9E8)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MYL9

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.

Alternate Names

LC2020 kDa myosin light chain, MLC2Myosin RLC, MRLC1MLC-2C, myosin regulatory light chain 1, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, myosin regulatory light polypeptide 9, myosin, light chain 9, regulatory, myosin, light polypeptide 9, regulatory, MYRL2MGC3505

Gene Symbol

MYL9

Additional MYL9 Products

Product Documents for MYL9 Antibody (4C9E8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MYL9 Antibody (4C9E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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