MYL9 [p Ser19] Antibody (6D8B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33577

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6D8B7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MYL9 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S19 of human MYL9 (NP_006088.2).

Sequence:
ATSNV

Modification

p Ser 19

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MYL9 [p Ser19] Antibody (6D8B7)

MYL9 [p Ser19] Antibody (6D8B7)

Western Blot: MYL9 [p Ser19] Antibody (6D8B7) [NBP3-33577] -

Western Blot: MYL9 [p Ser19] Antibody (6D8B7) [NBP3-33577] - Western blot analysis of lysates from HeLa cells, using MYL9 Rabbit mAb at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
MYL9 [p Ser19] Antibody (6D8B7)

Western Blot: MYL9 [p Ser19] Antibody (6D8B7) [NBP3-33577] -

Western Blot: MYL9 [p Ser19] Antibody (6D8B7) [NBP3-33577] - Western blot analysis of lysates from C2C12 cells, using MYL9 Rabbit mAb at 1:1000 dilution. C2C12 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

Applications for MYL9 [p Ser19] Antibody (6D8B7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MYL9

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.

Alternate Names

LC2020 kDa myosin light chain, MLC2Myosin RLC, MRLC1MLC-2C, myosin regulatory light chain 1, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, myosin regulatory light polypeptide 9, myosin, light chain 9, regulatory, myosin, light polypeptide 9, regulatory, MYRL2MGC3505

Gene Symbol

MYL9

Additional MYL9 Products

Product Documents for MYL9 [p Ser19] Antibody (6D8B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MYL9 [p Ser19] Antibody (6D8B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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