MYLK2 Antibody (2G1)
Novus Biologicals, part of Bio-Techne | Catalog # H00085366-M01
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2G1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MYLK2 (NP_149109, 161 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDC
Reactivity Notes
Human. Other species not tested.
Localization
Cytoplasmic
Specificity
MYLK2 - myosin light chain kinase 2, skeletal muscle
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for MYLK2 Antibody (2G1)
![Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]](https://resources.bio-techne.com/images/products/MYLK2-Antibody-2G1-Western-Blot-H00085366-M01-img0005.jpg "Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]")
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01] - The antibody was tested in mouse skeletal muscle and shows a band below 75 kDa. This image was submitted via customer Review. *{Mouse WB}*![Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]](https://resources.bio-techne.com/images/products/MYLK2-Antibody-2G1-Western-Blot-H00085366-M01-img0003.jpg "Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]")
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01] - Analysis of MYLK2 over-expressed 293 cell line, cotransfected with MYLK2 Validated Chimera RNAi ( Cat # H00085366-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYLK2 monoclonal antibody (M01), clone 2G1 (Cat # H00085366-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]](https://resources.bio-techne.com/images/products/MYLK2-Antibody-2G1-Western-Blot-H00085366-M01-img0004.jpg "Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]")
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01]
Western Blot: MYLK2 Antibody (2G1) [H00085366-M01] - Analysis of MYLK2 expression in transfected 293T cell line by MYLK2 monoclonal antibody (M01), clone 2G1.Lane 1: MYLK2 transfected lysate(64.7 KDa).Lane 2: Non-transfected lysate.Applications for MYLK2 Antibody (2G1)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It has also been used for RNAi validation.
Reviewed Applications
Read 1 review rated 5 using H00085366-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MYLK2
Long Name
Myosin light chain kinase 2, skeletal/cardiac muscle
Alternate Names
EC 2.7.11.18, MLCK2
Entrez Gene IDs
85366 (Human)
Gene Symbol
MYLK2
OMIM
192600 (Human)
UniProt
Additional MYLK2 Products
Product Documents for MYLK2 Antibody (2G1)
Product Specific Notices for MYLK2 Antibody (2G1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...