MyoD Antibody (RM369)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77430

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-77430
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM369

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MyoD Antibodies »

Product Summary for MyoD Antibody (RM369)

Immunogen

A peptide corresponding to residues near N-terminus of human MyoD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MyoD Antibody (RM369)

Immunohistochemistry-Paraffin: MyoD Antibody (RM369) [NBP2-77430]

Immunohistochemistry-Paraffin: MyoD Antibody (RM369) [NBP2-77430]

Immunohistochemistry-Paraffin: MyoD Antibody (RM369) [NBP2-77430] - Mouse skeletal muscle tissue lysate using anti-MyoD1 rabbit monoclonal antibody (Clone RM369) at a 1:2000 dilution.
MyoD Antibody (RM369)

Western Blot: MyoD Antibody (RM369) [NBP2-77430] -

Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.

Applications for MyoD Antibody (RM369)

Application
Recommended Usage

Immunohistochemistry

1:500-1:1000

Western Blot

1:1000 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (RM369)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MyoD Antibody (RM369)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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