MyoD Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54153
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]](https://resources.bio-techne.com/images/products/MyoD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0005.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Block/Neutralize, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A partial recombinant protein made to an N-terminal region of the human MyoD1 protein (within residues 1-150). [Swiss-Prot# P15172]
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28569745).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MyoD Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]
Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Western Blot: MyoD AntibodyBSA Free [NBP1-54153]](https://resources.bio-techne.com/images/products/MyoD1-Antibody-Western-Blot-NBP1-54153-img0004.jpg "Western Blot: MyoD AntibodyBSA Free [NBP1-54153]")
Western Blot: MyoD AntibodyBSA Free [NBP1-54153]
Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.![Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]](https://resources.bio-techne.com/images/products/MyoD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]")
Immunocytochemistry/ Immunofluorescence: MyoD Antibody - BSA Free [NBP1-54153]
Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).Applications for MyoD Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:5000
Application Notes
This MyoD1 antibody is useful for ICC/IF and Western blot, where a band is seen ~42 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MyoD
Long Name
Myoblast Determination Protein 1
Alternate Names
bHLHc1, MYF3, MYOD1, PUM
Gene Symbol
MYOD1
UniProt
Additional MyoD Products
Product Documents for MyoD Antibody - BSA Free
Product Specific Notices for MyoD Antibody - BSA Free
Manufactured by Genomic Antibody Technology™. GAT FAQs
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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