Myogenin Antibody (F12B)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30816

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # F12B

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myogenin Antibodies »

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.

Specificity

Cat#, clone F12B recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Myogenin Antibody (F12B)

Myogenin Antibody (F12B)

Immunocytochemistry/ Immunofluorescence: Myogenin Antibody (F12B) [NBP3-30816] -

Immunocytochemistry/ Immunofluorescence: Myogenin Antibody (F12B) [NBP3-30816] - Figure 2: Immunoreactivity of NBP3-30816, the mouse anti human myogenin in nuclei of formaldehyde fixed C2C12 mouse muscle myoblast cells in culture (high magnification).
Myogenin Antibody (F12B)

Immunohistochemistry-Paraffin: Myogenin Antibody (F12B) [NBP3-30816] -

Immunohistochemistry-Paraffin: Myogenin Antibody (F12B) [NBP3-30816] - Figure 1. Immunohistochemical staining using myogenin antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma.

Applications for Myogenin Antibody (F12B)

Application
Recommended Usage

Immunohistochemistry-Paraffin

: 1-2 ug/ml

Immunoprecipitation

: 2 ug/mg

Western Blot

: 1 ug/ml
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Phosphate buffered saline

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Myogenin

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Alternate Names

BHLHC3, MYF4, MYOG

Gene Symbol

MYOG

Additional Myogenin Products

Product Documents for Myogenin Antibody (F12B)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myogenin Antibody (F12B)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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