Myosin heavy chain 2 Antibody (JE53-30)

Western Blot: Myosin heavy chain 2 Antibody (JE53-30) [NBP3-32625]

Western Blot: Myosin heavy chain 2 Antibody (JE53-30) [NBP3-32625] - Western blot analysis of Myosin heavy chain 2 on different lysates with Rabbit anti-Myosin heavy chain 2 antibody (NBP3-32625) at 1/1,000 dilution.

Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 223 kDa
Observed band size: 223 kDa

Exposure time: 2 minutes 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32625) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Myosin heavy chain 2 Antibody (JE53-30) [NBP3-32625]

Immunohistochemistry: Myosin heavy chain 2 Antibody (JE53-30) [NBP3-32625] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Myosin heavy chain 2 antibody (NBP3-32625) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32625) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.