Myosin Light Chain 2 Antibody (3B9-B4)

Novus Biologicals, part of Bio-Techne | Catalog # H00004633-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B9-B4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Myosin Light Chain 2 Antibodies »

Product Specifications

Immunogen

MYL2 (AAH15821.1, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

Specificity

MYL2 - myosin, light polypeptide 2, regulatory, cardiac, slow

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Myosin Light Chain 2 Antibody (3B9-B4)

Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01]

Immunocytochemistry/ Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01]

Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of monoclonal antibody to MYL2 on HeLa cell . Antibody concentration 10 ug/ml.
Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01]

Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01]

Immunoprecipitation: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Analysis of MYL2 transfected lysate using anti-MYL2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYL2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Myosin Light Chain 2 Antibody (3B9-B4) [H00004633-M01] - Detection limit for recombinant GST tagged MYL2 is 0.03 ng/ml as a capture antibody.

Applications for Myosin Light Chain 2 Antibody (3B9-B4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myosin Light Chain 2

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Alternate Names

cardiac ventricular myosin light chain 2, CMH10myosin light chain 2, DKFZp779C0562, MLC2, MLC-2, MLC-2v, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, myosin, light chain 2, regulatory, cardiac, slow, myosin, light polypeptide 2, regulatory, cardiac, slow, regulatory light chain of myosin, RLC of myosin, slow cardiac myosin regulatory light chain 2

Entrez Gene IDs

4633 (Human)

Gene Symbol

MYL2

OMIM

160781 (Human)

UniProt

AAH15821 (Human)

Additional Myosin Light Chain 2 Products

Product Documents for Myosin Light Chain 2 Antibody (3B9-B4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myosin Light Chain 2 Antibody (3B9-B4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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