N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32397
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE64-81
Concentration
1 mg/ml
Product Summary for N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)
Immunogen
Synthetic peptide within human N-Acetylmannosamine Kinase/GNE aa 673-722/722. (Uniprot: Q9Y223)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)
Western Blot: N-Acetylmannosamine Kinase/GNE Antibody (JE64-81) [NBP3-32397]
Western Blot: N-Acetylmannosamine Kinase/GNE Antibody (JE64-81) [NBP3-32397] - Western blot analysis of N-Acetylmannosamine Kinase/GNE on different lysates with Rabbit anti-N-Acetylmannosamine Kinase/GNE antibody (NBP3-32397) at 1/500 dilution.Lane 1: SW480 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32397) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
Applications for N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)
Application
Recommended Usage
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: N-Acetylmannosamine Kinase/GNE
Long Name
Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine Kinase
Alternate Names
DMRV, GLCNE, GNE, IBM2, NAcetylmannosamine Kinase, Uae1
Gene Symbol
GNE
Additional N-Acetylmannosamine Kinase/GNE Products
Product Documents for N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)
Product Specific Notices for N-Acetylmannosamine Kinase/GNE Antibody (JE64-81)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...