N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94770

Novus Biologicals, part of Bio-Techne
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NBP2-94770-0.02ml
NBP2-94770-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all N-Acetylmannosamine Kinase/GNE Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GNE (NP_001121699.1). METYGYLQRESCFQGPHELYFKNLSKRNKQIMEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Western Blot: N-Acetylmannosamine Kinase/GNE AntibodyBSA Free [NBP2-94770]

Western Blot: N-Acetylmannosamine Kinase/GNE AntibodyBSA Free [NBP2-94770]

Western Blot: N-Acetylmannosamine Kinase/GNE Antibody [NBP2-94770] - Analysis of extracts of various cell lines, using N-Acetylmannosamine Kinase/GNE at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody - BSA Free [NBP2-94770]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody - BSA Free [NBP2-94770]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP2-94770] - Paraffin-embedded mouse brain using N-Acetylmannosamine Kinase/GNE .
Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody - BSA Free [NBP2-94770]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody - BSA Free [NBP2-94770]

Immunohistochemistry-Paraffin: N-Acetylmannosamine Kinase/GNE Antibody [NBP2-94770] - Paraffin-embedded human esophagus using N-Acetylmannosamine Kinase/GNE .

Applications for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N-Acetylmannosamine Kinase/GNE

The protein encoded by the GNE gene is a bifunctional enzyme that monitors and begins biosynthesis of N-acetylneuraminic acid (NeuAc). NeuAC is a precursor of sialic acids and functions in early development as sialylation is a component of cell adhesion, signal transduction, and tumorigencity and metastic behavior of malignant cells. The GNE gene has been linked to various diseases such as: myopathy, dementia, glomerulosclerosis, muscular dystrophy, and neuromuscular disease. This protein creates an enzyme that is rate-limiting in the sialic acid biosynthetic pathway as well as plays a role in amino and nucleotide sugar metabolism as well as CMP-N-acetylneuraminate biosynthesis in eukaryotes. The GNE gene is known to interact with genes CRMP1. ZBTB16, PIK3C2A, KIAA1549, and RIF1 to participate in these pathways. GNE exists is five isoforms: isoform 1: 722 amino acids long; 79 kDA; isoform 2: 753 amino acids long, 83 kDA; isoform 3: 681 amino acids long; 74 kDA; isoform 4: 648 amino acids long, 71 kDA; isoform 5: 612 amino acids long, 66 kDA.

Long Name

Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine Kinase

Alternate Names

DMRV, GLCNE, GNE, IBM2, NAcetylmannosamine Kinase, Uae1

Gene Symbol

GNE

Additional N-Acetylmannosamine Kinase/GNE Products

Product Documents for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for N-Acetylmannosamine Kinase/GNE Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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