N-Cadherin Antibody (OTI2G7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72891
![Western Blot: N-Cadherin Antibody (OTI2G7)Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Western-Blot-NBP2-72891-img0001.jpg "Western Blot: N-Cadherin Antibody (OTI2G7)Azide and BSA Free [NBP2-72891]")
![Western Blot: N-Cadherin Antibody (OTI2G7)Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Western-Blot-NBP2-72891-img0002.jpg "Western Blot: N-Cadherin Antibody (OTI2G7)Azide and BSA Free [NBP2-72891]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2G7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This N-Cadherin Antibody (OTI2G7) - Azide and BSA Free was developed against a full length recombinant protein of human CDH2(NP_001783) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for N-Cadherin Antibody (OTI2G7) - Azide and BSA Free
Western Blot: N-Cadherin Antibody (OTI2G7)Azide and BSA Free [NBP2-72891]
Western Blot: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY N Cadherin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-N Cadherin.![Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72891-img0007.jpg "Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]")
Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]
Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-N Cadherin mouse monoclonal antibody.![Flow Cytometry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72891-img0004.jpg "Flow Cytometry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]")
Flow Cytometry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]
Flow Cytometry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-N Cadherin antibody, and then analyzed by flow cytometry.![Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72891-img0005.jpg "Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]")
![Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72891-img0006.jpg "Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]")
![Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72891-img0008.jpg "Immunohistochemistry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]")
![Flow Cytometry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]](https://resources.bio-techne.com/images/products/N-Cadherin-Antibody-OTI2G7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72891-img0003.jpg "Flow Cytometry: N-Cadherin Antibody (OTI2G7) - Azide and BSA Free [NBP2-72891]")
Applications for N-Cadherin Antibody (OTI2G7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: N-Cadherin
N-cadherin is expressed on multiple cell types but is most highly expressed by mesenchymal cells and neural tissue (2). Functionally, N-cadherin has a number of roles including maintaining structural integrity and adhesion, cell signaling, and formation of neuronal synapses and the vascular wall (2). The cytoplasmic tail interacts with beta-catenin which then binds with alpha-catenin, forming the cadherin-catenin adhesion complex, an important component of adhesions junctions (1-3). Given its role in adhesion, N-cadherin serves as an indicator of epithelial-to-mesenchymal transition (EMT) (1-4). The loss of E-cadherin during EMT corresponds with an increase in N-cadherin expression (1-4). This "cadherin-switch" is associated with increased migratory and invasive behavior observed in tumor progress (1-4). Proteases including activity of a disintegrin and metalloprotease 10 (ADAM10), matrix metalloproteinases (MMPs), caspase 3, presenilin, and calpain can cleave N-cadherin as a mechanism for regulating Wnt/beta-catenin signaling and inducing oncogenic signals (3,4). In addition to its expression in solid tumors, N-cadherin has been indicated in hematological disorders such as leukemia and multiple myeloma (1). N-cadherin antagonists are currently being studied as potential therapeutics for a variety of cancer studies (1-2).
References
1. Mrozik, K. M., Blaschuk, O. W., Cheong, C. M., Zannettino, A., & Vandyke, K. (2018). N-cadherin in cancer metastasis, its emerging role in haematological malignancies and potential as a therapeutic target in cancer. BMC Cancer. https://doi.org/10.1186/s12885-018-4845-0
2. Loh, C. Y., Chai, J. Y., Tang, T. F., Wong, W. F., Sethi, G., Shanmugam, M. K., Chong, P. P., & Looi, C. Y. (2019). The E-Cadherin and N-Cadherin Switch in Epithelial-to-Mesenchymal Transition: Signaling, Therapeutic Implications, and Challenges. Cells. https://doi.org/10.3390/cells8101118
3. Derycke, L. D., & Bracke, M. E. (2004). N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling. The International Journal of Developmental Biology. https://doi.org/10.1387/ijdb.041793ld
4. Yu, W., Yang, L., Li, T., & Zhang, Y. (2019). Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target. Frontiers in Oncology. https://doi.org/10.3389/fonc.2019.00989
5. Unitprot (P1903)
Long Name
Neural Cadherin
Alternate Names
Cadherin-2, CD325, CDH2, NCadherin
Gene Symbol
CDH2
Additional N-Cadherin Products
Product Documents for N-Cadherin Antibody (OTI2G7) - Azide and BSA Free
Product Specific Notices for N-Cadherin Antibody (OTI2G7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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