N-WASP Antibody (5F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00008976-M04

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all N-WASP Antibodies »

Product Specifications

Immunogen

WASL (NP_003932, 97 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISH

Specificity

WASL - Wiskott-Aldrich syndrome-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for N-WASP Antibody (5F4)

Western Blot: N-WASP Antibody (5F4) [H00008976-M04]

Western Blot: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of WASL expression in IMR-32 (Cat # L008V1).

Immunocytochemistry/ Immunofluorescence: N-WASP Antibody (5F4) [H00008976-M04]

Immunocytochemistry/Immunofluorescence: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of monoclonal antibody to WASL on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: N-WASP Antibody (5F4) [H00008976-M04]

Immunohistochemistry-Paraffin: N-WASP Antibody (5F4) [H00008976-M04] - Analysis of monoclonal antibody to WASL on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.8 ug/ml

Applications for N-WASP Antibody (5F4)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: N-WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Entrez Gene IDs

8976 (Human)

Gene Symbol

WASL

OMIM

605056 (Human)

UniProt

NP_003932 (Human)

Additional N-WASP Products

Product Documents for N-WASP Antibody (5F4)

Product Datasheets

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COA

SDS

Product Specific Notices for N-WASP Antibody (5F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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