N-WASP [p Tyr256] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1169

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB600-1169
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Validated:

ELISA, Western Blot

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all N-WASP Antibodies »

Product Specifications

Immunogen

Phospho N WASP (Tyr 256) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around tyrosine 256 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding tyrosine 291.

Epitope

phosphotyrosine 256

Modification

p Tyr256

Localization

Cytoplasmic

Specificity

N WASP [Tyr256]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N-WASP [p Tyr256] Antibody

Western Blot: N-WASP [p Tyr256] Antibody [NB600-1169]

Western Blot: N-WASP [p Tyr256] Antibody [NB600-1169]

Western Blot: N-WASP [p Tyr256] Antibody [NB600-1169] - Western blot analysis of control and pervanadate-treated A431 cells (20 ug/lane). Blots were probed with either rabbit polyclonal anti-N-WASP (Ser484/Ser485) or anti-N-WASP (Tyr256).

Applications for N-WASP [p Tyr256] Antibody

Application
Recommended Usage

ELISA

1:20000

Western Blot

1:10000
Application Notes
In Western Blot a 65kDa band is seen. Optimal dilutions should be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1 ml PBS, 1.0 BSA mg/ml and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: N-WASP

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Entrez Gene IDs

8976 (Human)

Gene Symbol

WASL

Additional N-WASP Products

Product Documents for N-WASP [p Tyr256] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for N-WASP [p Tyr256] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.