NACA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00004666-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NACA1 Antibodies »

Product Specifications

Immunogen

NACA (NP_005585.1, 1 a.a. - 215 a.a.) full-length human protein. MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM

Specificity

NACA - nascent polypeptide-associated complex alpha subunit,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for NACA1 Antibody

Western Blot: NACA1 Antibody [H00004666-B01P]

Western Blot: NACA1 Antibody [H00004666-B01P]

Western Blot: NACA1 Antibody [H00004666-B01P] - Analysis of NACA expression in transfected 293T cell line by NACA polyclonal antibody. Lane 1: NACA transfected lysate(23.65 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NACA1 Antibody [H00004666-B01P]

Immunocytochemistry/ Immunofluorescence: NACA1 Antibody [H00004666-B01P]

Immunocytochemistry/Immunofluorescence: NACA1 Antibody [H00004666-B01P] - Analysis of purified antibody to NACA on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: NACA1 Antibody [H00004666-B01P]

Western Blot: NACA1 Antibody [H00004666-B01P]

Western Blot: NACA1 Antibody [H00004666-B01P] - analysis of NACA expression in transfected 293T cell line by NACA MaxPab polyclonal antibody. Lane 1: NACA transfected lysate (23.65 KDa). Lane 2: Non-transfected lysate.

Applications for NACA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NACA1

Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters

Alternate Names

Allergen Hom s 2, Alpha-NAC, MGC117224, NACA1, NAC-alpha, nascent polypeptide-associated complex alpha subunit, nascent polypeptide-associated complex subunit alpha, nascent-polypeptide-associated complex alpha polypeptide

Entrez Gene IDs

4666 (Human)

Gene Symbol

NACA

Additional NACA1 Products

Product Documents for NACA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NACA1 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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