NALP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31375

Novus Biologicals, part of Bio-Techne
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NBP2-31375
NBP2-31375SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NALP4 Antibodies »

Product Summary for NALP4 Antibody

Immunogen

Partial recombinant human NALP4 protein (between residues 1-300) [UniProt Q96MN2]

Reactivity Notes

Immunogen shows 51% similarity with Mouse's NALP4.

Localization

Cytoplasm

Specificity

Besides detecting canonical isoform (~113.4 kDa), this antibody may detect isoform 3 (104 kDa) also.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NALP4 Antibody

Western Blot: NALP4 Antibody [NBP2-31375]

Western Blot: NALP4 Antibody [NBP2-31375]

Western Blot: NALP4 Antibody [NBP2-31375] - WB detection of NALP4/ NLRP4 protein in (A) K562 cell lysate and (B) partial recombinant protein by using NALP4 antibody at a concentration of: 1 ug/ml for lysate and 0.05 ug/ml for recombinant protein. In K562 cells lysate, this antibody detected a single specific band at ~116 kDa position (predicted size of NALP4 is 113.4 kDa).
Immunocytochemistry/ Immunofluorescence: NALP4 Antibody [NBP2-31375]

Immunocytochemistry/ Immunofluorescence: NALP4 Antibody [NBP2-31375]

Immunocytochemistry/Immunofluorescence: NALP4 Antibody [NBP2-31375] - NALP4 antibody was tested in MCF7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.

Applications for NALP4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.1 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NALP4

NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP4, also known as PYRIN-containing APAF1-like protein 4, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. In transfected 293 cells, NALP4 suppressed NF-kappaB induction by TNF-alpha and IL-1beta, suggesting NALP4 operates at a point of convergence in these two signaling pathways. NALP4 also suppressed NF-kappaB induction resulting from overexpression of several adapter proteins and protein kinases involved in these pathways, including TRAF2, TRAF6, RIP, and IRAK2, as well as the IKK-alpha and IKK-beta, suggesting that NALP4 is critical in modulating NF-kappaB activity.

Alternate Names

Cancer/testis antigen 58, CLR19.5, CT58PYRIN and NACHT-containing protein 2, FLJ32126, NACHT, leucine rich repeat and PYD containing 4, NACHT, LRR and PYD domains-containing protein 4, NALP4NACHT, LRR and PYD containing protein 4, NLR family, pyrin domain containing 4, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 4, PAN2Ribonuclease inhibitor 2, PYPAF4PAAD and NACHT-containing protein 2, RNH2PYRIN-containing APAF1-like protein 4

Entrez Gene IDs

147945 (Human)

Gene Symbol

NLRP4

UniProt

Q96MN2 (Human)

Additional NALP4 Products

Product Documents for NALP4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NALP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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