Nanog Antibody (1E6C4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47427

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E6C4

Format

BSA Free

Concentration

1.0 mg/ml

View all Nanog Antibodies »

Product Summary for Nanog Antibody (1E6C4) - BSA Free

Immunogen

Purified recombinant fragment of human Nanog (amino acids 20-166) expressed in E. coli. [UniProt# Q9H9S0]

Localization

Nucleus

Marker

Embryonic Stem Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nanog Antibody (1E6C4) - BSA Free

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (1E6C4) - BSA Free [NBP1-47427]

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (1E6C4) - BSA Free [NBP1-47427]

Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]

Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]

Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).
Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]

Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]

Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Applications for Nanog Antibody (1E6C4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This Nanog (1E6C4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP1-47427 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

79923 (Human)

Gene Symbol

NANOG

UniProt

Q9H9S0 (Human)

Additional Nanog Products

Product Documents for Nanog Antibody (1E6C4) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nanog Antibody (1E6C4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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