Nanog Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-59737
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DLFLNYSMNMQPED corresponding to C-Terminus according to NP_079141.2.
Marker
Embryonic Stem Cell Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nanog Antibody
![Western Blot: Nanog Antibody [NB100-59737]](https://resources.bio-techne.com/images/products/Nanog-Antibody-Western-Blot-NB100-59737-img0007.jpg "Western Blot: Nanog Antibody [NB100-59737]")
Western Blot: Nanog Antibody [NB100-59737]
Western Blot: Nanog Antibody [NB100-59737] - Staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry: Nanog Antibody [NB100-59737]](https://resources.bio-techne.com/images/products/Nanog-Antibody-Immunohistochemistry-NB100-59737-img0001.jpg "Immunohistochemistry: Nanog Antibody [NB100-59737]")
Immunohistochemistry: Nanog Antibody [NB100-59737]
Immunohistochemistry: Nanog Antibody [NB100-59737] - IHC staining of paraffin embedded Human Testis using NB100-59737 at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Nanog Antibody
Application
Recommended Usage
Immunohistochemistry
1 - 2 ug/mL
Immunohistochemistry-Paraffin
1 - 2 ug/mL
Peptide ELISA
Detection limit 1:128000
Western Blot
0.05 - 0.1 ug/mL
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. This antibody is reported to work in Sandwich ELISA both as a capturer and as a reporter in combination with NB110-40660. WB: Approx. 35 kDa band observed in HeLa nuclear lysates (calculated MW of 34.6 kDa band according to NP_079141.1). An additional band of approx. 37 kDa band is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources. IHC-P: Human testis shows nuclear staining in spermatogonia.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Nanog
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Entrez Gene IDs
79923 (Human)
Gene Symbol
NANOG
UniProt
Additional Nanog Products
Product Documents for Nanog Antibody
Product Specific Notices for Nanog Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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