As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.

Name: Nanog Antibody
Brand: Novus Biologicals
SKU: NB110-40660

This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For Nanog Antibody NB110-40660, its immunogen is shown on its web page datasheet. The result of the UniProt Blast was a 93% match to both F6PPY5 and F6PXN9. 93% means high homology, and suggests that this antibody would be suitable for horse samples, although we cannot guarantee it. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.

Nanog Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40660

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Citations (8)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine

Cited:

Human, Mouse, Porcine

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Nanog Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QNQRMKSKRWQKNN corresponding to internal region according to NP_079141.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21678472). Use in Porcine reported in scientific literature (PMID:21685711).

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nanog Antibody

Western Blot: Nanog Antibody [NB110-40660]

Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB110-40660]

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.

Applications for Nanog Antibody

Application

Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/mL

Application Notes

WB: Approx. 38 kDa band observed in human ovary lysates (calculated MW of 34.6 kDa band according to NP_079141.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nanog

Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Nanog is a 304 amino acid polypeptide which lacks a signal sequence for secretion.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

79923 (Human); 71950 (Mouse); 414065 (Rat)

Gene Symbol

NANOG

UniProt

Q9H9S0 (Human)

Additional Nanog Products

Product Documents for Nanog Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Nanog Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

Nanog Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40660

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all Nanog Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Marker

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for Nanog Antibody

Western Blot: Nanog Antibody [NB110-40660]

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB110-40660]

Applications for Nanog Antibody

Peptide ELISA

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Nanog

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Nanog Products

Product Documents for Nanog Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Nanog Antibody