Nanog Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77109
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Chicken (94%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG. Amino Acid Squence: KQVKTWFQNQRMKSKRWQK
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (89%)
Marker
Embryonic Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nanog Antibody - BSA Free
Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] -
Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] -
Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Mouse Testis.Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] -
Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Human Testis.Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Nanog antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Applications for Nanog Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nanog
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Gene Symbol
NANOG
UniProt
Additional Nanog Products
Product Documents for Nanog Antibody - BSA Free
Product Specific Notices for Nanog Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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