Nanog Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24941
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Primate (96%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 1-50 of human NANOG was used as the immunogen.
Marker
Embryonic Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nanog Antibody
Western Blot: Nanog Antibody [NBP2-24941]
Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP2-24941]
Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP2-24941] - Nanog antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.Western Blot: Nanog Antibody [NBP2-24941]
Western Blot: Nanog Antibody [NBP2-24941] - Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. MM - molecular weight marker, C - cells obtained from control cows, ADENO - cells obtained from cows with adenomyosis. Image collected and cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/s12958-015-0106-0), licensed under a CC-BY license.Applications for Nanog Antibody
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 26283864)
Immunocytochemistry/ Immunofluorescence
1:10
Western Blot
0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nanog
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Gene Symbol
NANOG
UniProt
Additional Nanog Products
Product Documents for Nanog Antibody
Product Specific Notices for Nanog Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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