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Nanog Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24941

Novus Biologicals, part of Bio-Techne
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NBP2-24941
NBP2-24941SS

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Primate (96%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A portion of amino acids 1-50 of human NANOG was used as the immunogen.

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nanog Antibody

Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP2-24941]

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP2-24941]

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP2-24941] - Nanog antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.
Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941] - Protein expression of pluripotency markers in uterine cells isolated from control cows and from cows with adenomyosis. MM - molecular weight marker, C - cells obtained from control cows, ADENO - cells obtained from cows with adenomyosis. Image collected and cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/s12958-015-0106-0), licensed under a CC-BY license.

Applications for Nanog Antibody

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 26283864)

Immunocytochemistry/ Immunofluorescence

1:10

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Gene Symbol

NANOG

UniProt

Additional Nanog Products

Product Documents for Nanog Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nanog Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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