NanoLuc® (Nluc) Luciferase Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB100261
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Capture, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 965853
Product Specifications
Immunogen
Synthetic peptide for NanoLuc® (Nluc) Luciferase
Specificity
Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. In Western blots, no cross-reactivity with Large BiT is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for NanoLuc® (Nluc) Luciferase Antibody
Detection of NanoLuc® Luciferase by Western Blot.
Western blot shows Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB100261) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for NanoLuc® (Nluc) Luciferase Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Recombinant NanoLuc®
Sample: Recombinant NanoLuc®
Reviewed Applications
Read 1 review rated 5 using MAB100261 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Luciferase
Alternate Names
LuC, luciferin 4 monooxygenase, Luciferin 4-monooxygenase
Additional Luciferase Products
Product Documents for NanoLuc® (Nluc) Luciferase Antibody
Product Specific Notices for NanoLuc® (Nluc) Luciferase Antibody
NanoLuc and NanoBiT are registered trademarks of Promega Corporation.
For research use only
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