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NBR1 Antibody [DyLight 650]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71703C

Novus Biologicals, part of Bio-Techne
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NBP1-71703C

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA Capture, Simple Western, Western Blot

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for NBR1 Antibody [DyLight 650]

Immunogen

A genomic peptide made to an internal region of the human NBR1 protein (within residues 50-250). [Swiss-Prot Q14596]

Reactivity Notes

Immunogen has 75% identity to the rat protein.

Localization

Cytoplasm. Cytoplasmic vesicle - autophagosome. Lysosome. Cytoplasm - myofibril - sarcomere - M line. Note: In cardiac muscles localizes to the sarcomeric M line. Is targeted to lysosomes for degradation.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for NBR1 Antibody [DyLight 650]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA Capture

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NBR1

NBR1 is a cargo receptor that sequesters ubiquinated proteins in the same manner as p62/SQSTM1, which leads to selective autophagy, sequestering the proteins into autophagomes. Both NBR1 and p62 localize to the endoplasmic reticulum independently of LC3. When the ability of cells to induce autophagy is impaired, aggregation of ubiquinated proteins occurs, and causes certain diseases. Particularly in neurodegenerative diseases, the functionality of NBR1 is adversely affected, which is related to the formation of protein aggregations within the brain. Analysis of the location and abundance of NBR1 in tissues can be a good indicator of autophagic failure and disease.

Alternate Names

Cell migration-inducing gene 19 protein, FLJ98272, KIAA0049CA125, M17S2FLJ55359,1A1-3B, Membrane component chromosome 17 surface marker 2,1A13B, membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigenCA125), migration-inducing protein 19, neighbor of BRCA1 gene 1, Neighbor of BRCA1 gene 1 protein, next to BRCA1 gene 1 protein, Protein 1A1-3B

Gene Symbol

NBR1

Additional NBR1 Products

Product Documents for NBR1 Antibody [DyLight 650]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NBR1 Antibody [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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