NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52669

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52669-0.025mg
NBP2-52669-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 735

Format

Azide and BSA Free

Concentration

1 mg/ml

View all NCAM-1/CD56 Antibodies »

Product Specifications

Immunogen

Homopolymers of alpha 2,8-linked sialic acid of Escherichia coli K1.

Specificity

Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of (alpha)2,8-linked sialic acid of Escherichia coli K1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free

Western Blot: NCAM-1/CD56 Antibody (735)ChimericAzide and BSA Free [NBP2-52669]

Western Blot: NCAM-1/CD56 Antibody (735)ChimericAzide and BSA Free [NBP2-52669]

Western Blot: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with NBP2-52669 at 2 ug/ml before being detected by a secondary antibody.
Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free [NBP2-52669]

Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free [NBP2-52669]

Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed rat spinal chord slices were were stained with a NBP2-52669 at 3 ug/ml.
Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free [NBP2-52669]

Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free [NBP2-52669]

Immunohistochemistry: NCAM-1/CD56 Antibody (735) - Chimeric [NBP2-52669] - Formalin fixed guinea pig brain slices were were stained with a NBP2-52669 at 3 ug/ml.

Applications for NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al. 1990). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al. 2006).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: NCAM-1/CD56

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

Additional NCAM-1/CD56 Products

Product Documents for NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCAM-1/CD56 Antibody (735) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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