NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47826
![Western Blot: NCAM-1/CD56 Antibody (NCAM1/795)Azide and BSA Free [NBP2-47826]](https://resources.bio-techne.com/images/products/NCAM-1-CD56-Antibody-NCAM1-795-Azide-and-BSA-Free-Western-Blot-NBP2-47826-img0001.jpg "Western Blot: NCAM-1/CD56 Antibody (NCAM1/795)Azide and BSA Free [NBP2-47826]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # NCAM1/795
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free
Immunogen
Recombinant human NCAM-1/CD56 protein
Localization
Cell surface
Specificity
This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Marker
Neuronal Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44717).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free
Western Blot: NCAM-1/CD56 Antibody (NCAM1/795)Azide and BSA Free [NBP2-47826]
Western Blot: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826] - Analysis of HCT116 Cell Lysate using CD56 Monoclonal Antibody (NCAM1/795)![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826]](https://resources.bio-techne.com/images/products/NCAM-1-CD56-Antibody-NCAM1-795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47826-img0004.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826]")
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826]
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826] - Human Lung Carcinoma stained with CD56 Monoclonal Antibody (NCAM1/795)![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826]](https://resources.bio-techne.com/images/products/NCAM-1-CD56-Antibody-NCAM1-795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47826-img0003.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826]")
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826]
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free [NBP2-47826] - Formalin-fixed, paraffin-embedded human Pancreas stained with NCAM-1/CD56 Antibody (NCAM1/795).Applications for NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: NCAM-1/CD56
Long Name
Neural Cell Adhesion Molecule
Alternate Names
CD56, NCAM1
Gene Symbol
NCAM1
UniProt
Additional NCAM-1/CD56 Products
Product Documents for NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free
Product Specific Notices for NCAM-1/CD56 Antibody (NCAM1/795) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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