NCAM-1/CD56 Antibody (SPM128)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34349

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-34349-0.02mg
NBP2-34349-0.1mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat, Zebrafish

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM128

Concentration

0.2 mg/ml

View all NCAM-1/CD56 Antibodies »

Product Specifications

Immunogen

Membrane preparation of a small cell lung carcinoma

Localization

Cell surface

Specificity

This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Marker

Neuronal Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34397)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NCAM-1/CD56 Antibody (SPM128)

Simple Western: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349]

Simple Western: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349]

Simple Western: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Detection of NCAM-1/CD56 by Simple WesternTM. Simple Western lane view shows lysates of human cerebellum. A specific band was detected for NCAM-1/CD56 at approximately 147 kDa (as indicated) using 10 ug/mL of Mouse Anti-NCAM-1/CD56 Monoclonal Antibody (Catalog # NBP2-34397). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349]

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-fixed, paraffin-embedded human Pancreas stained with NCAM-1/CD56 Antibody (SPM128)
Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349]

Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349]

Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - FACS analysis of CD56 on human Monocytes using NCAM-1/CD56 Antibody (SPM128)
View 1 more images

Applications for NCAM-1/CD56 Antibody (SPM128)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NCAM-1/CD56

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Long Name

Neural Cell Adhesion Molecule

Alternate Names

CD56, NCAM1

Gene Symbol

NCAM1

UniProt

P13592 (Human), P13591 (Rat)

Additional NCAM-1/CD56 Products

Product Documents for NCAM-1/CD56 Antibody (SPM128)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NCAM-1/CD56 Antibody (SPM128)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.