NCK1 Antibody (1A1)
Novus Biologicals, part of Bio-Techne | Catalog # H00004690-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1A1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NCK1 (AAH06403, 185 a.a. ~ 294 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEM
Localization
Cytoplasmic
Specificity
NCK1 - NCK adaptor protein 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NCK1 Antibody (1A1)
![Western Blot: NCK1 Antibody (1A1) [H00004690-M01]](https://resources.bio-techne.com/images/products/NCK1-Antibody-1A1-Western-Blot-H00004690-M01-img0005.jpg "Western Blot: NCK1 Antibody (1A1) [H00004690-M01]")
Western Blot: NCK1 Antibody (1A1) [H00004690-M01]
Western Blot: NCK1 Antibody (1A1) [H00004690-M01] - Analysis of NCK1 expression in transfected 293T cell line by NCK1 monoclonal antibody (M01), clone 1A1.Lane 1: NCK1 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.![Western Blot: NCK1 Antibody (1A1) [H00004690-M01]](https://resources.bio-techne.com/images/products/NCK1-Antibody-1A1-Western-Blot-H00004690-M01-img0004.jpg "Western Blot: NCK1 Antibody (1A1) [H00004690-M01]")
Western Blot: NCK1 Antibody (1A1) [H00004690-M01]
Western Blot: NCK1 Antibody (1A1) [H00004690-M01] - NCK1 monoclonal antibody (M01), clone 1A1 Analysis of NCK1 expression in Hela S3 NE.![ELISA: NCK1 Antibody (1A1) [H00004690-M01]](https://resources.bio-techne.com/images/products/NCK1-Antibody-1A1-ELISA-H00004690-M01-img0006.jpg "ELISA: NCK1 Antibody (1A1) [H00004690-M01]")
ELISA: NCK1 Antibody (1A1) [H00004690-M01]
ELISA: NCK1 Antibody (1A1) [H00004690-M01] - Detection limit for recombinant GST tagged NCK1 is approximately 0.1ng/ml as a capture antibody.Applications for NCK1 Antibody (1A1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NCK1
Long Name
Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1
Alternate Names
cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha
Entrez Gene IDs
4690 (Human)
Gene Symbol
NCK1
OMIM
600508 (Human)
UniProt
Additional NCK1 Products
Product Documents for NCK1 Antibody (1A1)
Product Specific Notices for NCK1 Antibody (1A1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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