NCK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19489

Novus Biologicals, part of Bio-Techne
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NBP2-19489
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCK1 Antibodies »

Product Summary for NCK1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCK1. The exact sequence is proprietary.

Localization

Cytoplasm; Endoplasmic reticulum

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCK1 Antibody

Western Blot: NCK1 Antibody [NBP2-19489]

Western Blot: NCK1 Antibody [NBP2-19489]

Western Blot: Nck Antibody [NBP2-19489] - Sample (30 ug of whole cell lysate) A: K562 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody [NBP2-19489]

Immunocytochemistry/ Immunofluorescence: NCK1 Antibody [NBP2-19489]

Immunocytochemistry/Immunofluorescence: Nck Antibody [NBP2-19489] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: NCK1 Antibody [NBP2-19489]

Immunohistochemistry-Paraffin: NCK1 Antibody [NBP2-19489]

Immunohistochemistry-Paraffin: Nck Antibody [NBP2-19489] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.

Applications for NCK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NCK1

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq]

Long Name

Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1

Alternate Names

cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha

Gene Symbol

NCK1

UniProt

P16333 (Human)

Additional NCK1 Products

Product Documents for NCK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NCK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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