NCK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82592

Novus Biologicals, part of Bio-Techne
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NBP1-82592
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCK2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCK2 Antibody

Western Blot: NCK2 Antibody [NBP1-82592]

Western Blot: NCK2 Antibody [NBP1-82592]

Western Blot: NCK2 Antibody [NBP1-82592] - Analysis in control (vector only transfected HEK293T lysate) and NCK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: NCK2 Antibody [NBP1-82592]

Immunohistochemistry-Paraffin: NCK2 Antibody [NBP1-82592]

Immunohistochemistry-Paraffin: NCK2 Antibody [NBP1-82592] - Staining of human skin shows strong cytoplasmic positivity in subsets of cells.

Applications for NCK2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCK2

NCK2 encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Alternate Names

GRB4, NCK adaptor protein 2cytoplasmic protein NCK2, Nck-2, NCKbeta, noncatalytic region of tyrosine kinase, beta, SH2/SH3 adaptor protein NCK-beta

Gene Symbol

NCK2

Additional NCK2 Products

Product Documents for NCK2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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