NCKAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32339

Novus Biologicals, part of Bio-Techne
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NBP1-32339
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (100%), Xenopus (96%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NCKAP1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NCKAP1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (96%).

Localization

Cell membrane, Single-pass membrane protein, Cytoplasmic side, Cell projection, lamellipodium membrane, Single-pass membrane protein, Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCKAP1 Antibody

Western Blot: NCKAP1 Antibody [NBP1-32339]

Western Blot: NCKAP1 Antibody [NBP1-32339]

Western Blot: NCKAP1 Antibody [NBP1-32339] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP1-32339]

Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP1-32339]

Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP1-32339] - Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody [C1C2], Internal diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: NCKAP1 Antibody [NBP1-32339]

Western Blot: NCKAP1 Antibody [NBP1-32339]

Western Blot: NCKAP1 Antibody [NBP1-32339] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.
View 1 more images

Applications for NCKAP1 Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NCKAP1

Is part of lamellipodial complex that controls Rac-dependent actin remodeling

Alternate Names

FLJ11291, HEM2p125Nap1, KIAA0587, Membrane-associated protein HEM-2, MGC8981, NAP 1, NAP125, NAP1Nap1, NCK-associated protein 1

Entrez Gene IDs

10787 (Human); 50884 (Mouse)

Gene Symbol

NCKAP1

UniProt

Q9Y2A7 (Human)

Additional NCKAP1 Products

Product Documents for NCKAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NCKAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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