NCKAP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83269
![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]](https://resources.bio-techne.com/images/products/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83269-img0009.jpg "Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]")
![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]](https://resources.bio-techne.com/images/products/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83269-img0008.jpg "Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCKAP1 Antibody
![Immunocytochemistry/ Immunofluorescence: NCKAP1 Antibody [NBP1-83269]](https://resources.bio-techne.com/images/products/NCKAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83269-img0006.jpg "Immunocytochemistry/ Immunofluorescence: NCKAP1 Antibody [NBP1-83269]")
Immunocytochemistry/ Immunofluorescence: NCKAP1 Antibody [NBP1-83269]
Immunocytochemistry/Immunofluorescence: NCKAP1 Antibody [NBP1-83269] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody shown in green.![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]](https://resources.bio-techne.com/images/products/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83269-img0007.jpg "Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]")
Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269]
Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP1-83269] - Staining of human cerebral cortex shows high expression.Applications for NCKAP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NCKAP1
Alternate Names
FLJ11291, HEM2p125Nap1, KIAA0587, Membrane-associated protein HEM-2, MGC8981, NAP 1, NAP125, NAP1Nap1, NCK-associated protein 1
Gene Symbol
NCKAP1
Additional NCKAP1 Products
Product Documents for NCKAP1 Antibody
Product Specific Notices for NCKAP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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