NCKAP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19491
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Rat (97%), Rhesus Macaque (100%), Xenopus (94%), Zebrafish (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human NCKAP1. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (94%).
Localization
Cell membrane; Single-pass membrane protein; Cytoplasmic side (By similarity); Cell projection; lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side (By similarity)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NCKAP1 Antibody
![Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491]](https://resources.bio-techne.com/images/products/NCKAP1-Antibody-Immunohistochemistry-Frozen-NBP2-19491-img0003.jpg "Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491]")
Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491]
Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491] - NCKAP1 Antibody detects NCKAP1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491]](https://resources.bio-techne.com/images/products/NCKAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-19491-img0002.jpg "Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491]")
Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491]
Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491] - Human lung Papillary adenocarcinoma, using NCKAP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NCKAP1 Antibody [NBP2-19491] -
Western Blot: NCKAP1 Antibody [NBP2-19491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term (NBP2-19491) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for NCKAP1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NCKAP1
Alternate Names
FLJ11291, HEM2p125Nap1, KIAA0587, Membrane-associated protein HEM-2, MGC8981, NAP 1, NAP125, NAP1Nap1, NCK-associated protein 1
Gene Symbol
NCKAP1
UniProt
Additional NCKAP1 Products
Product Documents for NCKAP1 Antibody
Product Specific Notices for NCKAP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.