NCOA3/AIB1 Antibody (D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-41658

Novus Biologicals, part of Bio-Techne
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NBP3-41658
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Key Product Details

Species Reactivity

Human, Porcine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # D3

Concentration

1 mg/ml

View all NCOA3 Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Human NCOA3/AIB1 (Uniprot#: Q9Y6Q9; Gly1023-Ala1304 (Uniprot#: Q9Y6Q9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Applications for NCOA3/AIB1 Antibody (D3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-20ug/mL

Western Blot

0.5-2ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCOA3

NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Gene Symbol

NCOA3

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody (D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCOA3/AIB1 Antibody (D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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