Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NCOA3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide, which represented a portion of human nuclear receptor coactivator 3 encoded within exon 16 (LocusLink ID 8202).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA3/AIB1 Antibody

Western Blot: NCOA3/AIB1 Antibody [NB100-315]

Western Blot: NCOA3/AIB1 Antibody [NB100-315]

Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 (SRC3) by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: NCOA3/AIB1 Antibody [NB100-315]

Western Blot: NCOA3/AIB1 Antibody [NB100-315]

Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: NCOA3/AIB1 Antibody [NB100-315]

Immunoprecipitation: NCOA3/AIB1 Antibody [NB100-315]

Immunoprecipitation: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 (lot 2) used for IP at 6 ug per reaction. NCOA3/AIB1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated NCOA3/AIB1, NB100-315 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for NCOA3/AIB1 Antibody

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
This protein is a cytoplasmic coactivator that shifts to the nucleus upon activation. It can be analyzed on Western blot using nuclear or cytosolic extracts.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA3

Steroid receptor coactivator 3 (SRC3) is a ~154 kDa nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in hormone-dependent fashion. SRC3 recruits 2 other nuclear factors, CBP and PCAF, and thus plays a central role in creating a multisubunit coactivator complex.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Entrez Gene IDs

8202 (Human)

Gene Symbol

NCOA3

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NCOA3/AIB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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