NCOA4 Antibody (1B7)
Novus Biologicals, part of Bio-Techne | Catalog # H00008031-M04
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NCOA4 (NP_005428, 505 a.a. ~ 614 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQM
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
NCOA4 (1B7)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NCOA4 Antibody (1B7)
![Western Blot: NCOA4 Antibody (1B7) [H00008031-M04]](https://resources.bio-techne.com/images/products/NCOA4-Antibody-1B7-Western-Blot-H00008031-M04-img0005.jpg "Western Blot: NCOA4 Antibody (1B7) [H00008031-M04]")
Western Blot: NCOA4 Antibody (1B7) [H00008031-M04]
Western Blot: NCOA4 Antibody (1B7) [H00008031-M04] - Analysis of NCOA4 expression in NIH/3T3 (Cat # L018V1).![Immunocytochemistry/ Immunofluorescence: NCOA4 Antibody (1B7) [H00008031-M04]](https://resources.bio-techne.com/images/products/NCOA4-Antibody-1B7-Immunocytochemistry-Immunofluorescence-H00008031-M04-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NCOA4 Antibody (1B7) [H00008031-M04]")
Immunocytochemistry/ Immunofluorescence: NCOA4 Antibody (1B7) [H00008031-M04]
Immunocytochemistry/Immunofluorescence: NCOA4 Antibody (1B7) [H00008031-M04] - Analysis of monoclonal antibody to NCOA4 on NIH/3T3 cell. Antibody concentration 10 ug/ml![ELISA: NCOA4 Antibody (1B7) [H00008031-M04]](https://resources.bio-techne.com/images/products/NCOA4-Antibody-1B7-ELISA-H00008031-M04-img0003.jpg "ELISA: NCOA4 Antibody (1B7) [H00008031-M04]")
ELISA: NCOA4 Antibody (1B7) [H00008031-M04]
ELISA: NCOA4 Antibody (1B7) [H00008031-M04] - Detection limit for recombinant GST tagged NCOA4 is approximately 3ng/ml as a capture antibody.Applications for NCOA4 Antibody (1B7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NCOA4
Alternate Names
Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator
Entrez Gene IDs
8031 (Human)
Gene Symbol
NCOA4
OMIM
188550 (Human)
UniProt
Additional NCOA4 Products
Product Documents for NCOA4 Antibody (1B7)
Product Specific Notices for NCOA4 Antibody (1B7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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