NCOA5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2570

Novus Biologicals, part of Bio-Techne
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NB100-2570
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NCOA5 Antibodies »

Product Summary for NCOA5 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Nuclear Receptor Coactivator 5 (Coactivator Independent of AF-2) using the numbering given in entry NP_066018.1 (GeneID 57727).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA5 Antibody

Western Blot: NCOA5 Antibody [NB100-2570]

Western Blot: NCOA5 Antibody [NB100-2570]

Western Blot: NCOA5 Antibody [NB100-2570] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA5 (CIA) antibody BL2431 used for WB at 0.04 ug/ml (A) and at 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). NCOA5 (CIA) was also immunoprecipitated by rabbit anti-NCOA5 (CIA) antibody BL2432. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2570]

Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2570]

Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2570] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- NCOA5 (CIA) used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for NCOA5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA5

NCOA5 is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c-myc in response to 17-beta-estradiol (E2).

Alternate Names

CIAbA465L10.6, Coactivator independent of AF-2, KIAA1637, NCoA-5, nuclear receptor coactivator 5

Entrez Gene IDs

57727 (Human)

Gene Symbol

NCOA5

UniProt

NP_066018.1 (Human)

Additional NCOA5 Products

Product Documents for NCOA5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NCOA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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