NCOR1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-58824
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunoprecipitation, Western Blot
Cited:
Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1525 and 1575 of human nuclear receptor co-repressor 1 using the numbering given in entry NP_006302.2 (GeneID 9611).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-347
Scientific Data Images for NCOR1 Antibody
![Western Blot: NCOR1 Antibody [NB100-58824]](https://resources.bio-techne.com/images/products/NCOR1-Antibody-Western-Blot-NB100-58824-img0002.jpg "Western Blot: NCOR1 Antibody [NB100-58824]")
Western Blot: NCOR1 Antibody [NB100-58824]
Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
![Immunoprecipitation: NCOR1 Antibody [NB100-58824]](https://resources.bio-techne.com/images/products/NCOR1-Antibody-Immunoprecipitation-NB100-58824-img0001.jpg "Immunoprecipitation: NCOR1 Antibody [NB100-58824]")
Immunoprecipitation: NCOR1 Antibody [NB100-58824]
Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.Applications for NCOR1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
10 ug
Chromatin Immunoprecipitation Sequencing
2 ug
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
ChIP-Seq 10 ug as per Ram O et al., Cell. 2011 Dec 23;147(7):1628-39.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NCOR1
Long Name
Nuclear Receptor Co-repressor 1
Alternate Names
KIAA1047, N-CoR, TRAC1
Entrez Gene IDs
9611 (Human)
Gene Symbol
NCOR1
UniProt
Additional NCOR1 Products
Product Documents for NCOR1 Antibody
Product Specific Notices for NCOR1 Antibody
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB200-347
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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