NCOR2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-5802

Novus Biologicals, part of Bio-Techne
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NB120-5802
NB120-5802-0.025mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all NCOR2 Antibodies »

Product Summary for NCOR2 Antibody

Immunogen

His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Localization

Nuclear

Specificity

NB120-5802 detects silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) from human cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOR2 Antibody

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry: NCOR2 Antibody [NB120-5802]

Immunohistochemistry: NCOR2 Antibody [NB120-5802]

Immunohistochemistry: NCOR2 Antibody [NB120-5802] - Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Human lung tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/ Immunofluorescence: NCOR2 Antibody [NB120-5802]

Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).
View 3 more images

Applications for NCOR2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

4 ug/ml
Application Notes
WB: Use at a concentration of 4 ug/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 302 kDa) representing SMRT from HeLa cell nuclear extracts.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCOR2

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.

Long Name

Nuclear Receptor Co-repressor 2

Alternate Names

CTG26, SMRT, SMRTE, TNRC14, TRAC-1

Entrez Gene IDs

9612 (Human)

Gene Symbol

NCOR2

Additional NCOR2 Products

Product Documents for NCOR2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NCOR2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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