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NDC80 Antibody (4W2Y2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16121

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16121-100ul
NBP3-16121-20ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4W2Y2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for NDC80 Antibody (4W2Y2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 543-642 of human NDC80 (O14777). ESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDC80 Antibody (4W2Y2)

Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121]

Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121]

Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121] - Western blot analysis of extracts of various cell lines, using NDC80 Rabbit mAb (NBP3-16121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Applications for NDC80 Antibody (4W2Y2)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDC80

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Alternate Names

HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), hsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3

Gene Symbol

NDC80

Additional NDC80 Products

Product Documents for NDC80 Antibody (4W2Y2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NDC80 Antibody (4W2Y2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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